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Protein Electrophoresis Urinalysis

What Is Protein Electrophoresis?

Proteins play an important role in supporting optimal body functioning and health. For example, they:

  • carry oxygen to where it’s needed in the body
  • aid in digestion
  • support muscle movement
  • fight off disease
  • support the growth and maintenance of tissues throughout the entire body

Proteins are found in the blood of healthy people. However, you shouldn’t have a lot of protein in your urine. Urine protein electrophoresis (UPEP) is a test your doctor can use to determine if there’s protein in your urine. It can also help your doctor find out how much of each type of protein is present.

Your doctor will usually order a UPEP test if they suspect that you have a condition that causes elevated protein levels in urine, such as multiple sclerosis or nephrotic syndrome.

Your doctor may also order this test if an earlier test showed there was protein in your urine. In this case, your doctor will use UPEP to monitor any treatment or disease progress.

Measurements That Can Be Determined Through UPEP

Most of the proteins in your body that help keep you healthy can be placed into two major groups: albumin and globulins.

Albumin is a single protein that is found in high levels in the blood.

Most globulins are produced in the liver. They include four main types of proteins:

  • alpha-1 globulin
  • alpha-2 globulin
  • beta globulin
  • gamma globulin

UPEP measures the levels of both major types of proteins in the urine.

Preparing for and Completing a UPEP Test

Test Preparation

Most people won’t need to take special steps to prepare for a UPEP test. However, you may need to stop taking some types of medication that can alter your test results. Examples include:

  • salicylates
  • chlorpromazine
  • corticosteroids
  • neomycin
  • tolbutamide

Ask your doctor before you stop taking any medications.

Completing the Test

To complete a UPEP test, you will need to collect a small amount of urine in the container provided.

Make sure your genital area is clean. Men should wipe the head of their penis. Women should use soap and water or an antibacterial wipe to clean their vulva. If using soap, make sure you fully rinse it away. This process is called a "clean-catch" specimen.

Urinate a small amount before you begin collecting the sample. This will help flush out impurities from the urethra so they won’t be included in the sample.

You will probably need to collect about 2 ounces of urine at most. Your doctor or a lab worker will tell you how much urine is needed.

Different methods are used for collecting urine samples from infants. Ask your doctor about urine collection bags and how to use them on your baby.

Overview of the Work Done in the Lab

Electrophoresis is used to analyze the urine sample. In this process, a lab technician places the urine on a special type of paper and applies an electric current. This causes the two main types of protein to separate and create bands on the paper. A lab technician analyzes the bands to determine if — and how much — proteins are present in the sample.

Interpreting Test Results

Normal Results

Interpreting the test results for UPEP is fairly straightforward. Globulins should not be present in your urine. Albumin levels should be below 5 milligrams per deciliter (mg/dL). Any amount of globulins or more than 5 mg/dL of albumin will be considered abnormal.

Elevated Protein Levels

A number of conditions can lead to high levels of protein in your urine. Examples include:

  • reduced kidney function or kidney failure
  • inflammation (acute as opposed to chronic)
  • urinary tract infection (acute)
  • kidney damage (may be due to a variety of causes)
  • multiple myeloma (a rare type of cancer)

Your doctor may need to perform other tests to pinpoint the exact cause of abnormal protein levels. After your doctor identifies the cause, they can provide more information about potential treatment options.

Content licensed from:

Written by: Krista O'Connell
Medically reviewed on: Jan 08, 2016: Steven Kim, MD

This feature is for informational purposes only and should not be used to replace the care and information received from your health care provider. Please consult a health care professional with any health concerns you may have.
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